Mfg Part Number
Ab01061-1.159-BS
Vector Labs Ab01061-1.159-BS Anti-CD47 [A4], Mouse IgG1-Fc Fusion, (1 mg)
Quick Overview
This mouse CD47-specific antagonist nanobody was raised by immunising alpacas with the extracellular Ig-like V-type domain (ECD) of mouse CD47. Subsequently, peripheral blood lymphocytes (PBLs) were isolated from immunized alpacas, and the extracted RNA was used to construct a VHH phage display library. The antibody was generated by panning against the mouse CD47 ECD.
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| Product Class: | Purified |
|---|---|
| Clone ID: | A4 |
| Synonyms: | IAP; CD47 antigen; CD47 glycoprotein; Integrin-associated protein; Leukocyte surface antigen CD47; MER6; OA3; Rh-related antigen |
| Amount: | 1 mg |
| Application Codes Clone: | antagonist; FC; blocking; functional assays; in vivo; in vitro |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS only. |
| Original Format: | VHH |
| Storage Temperature: | Store at 4⁰C for up to 3 months. Note, this antibody is provided without added preservatives, it is therefore recommed this antibody be handled under sterile conditions. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This chimeric mouse antibody was made using the variable domain sequences of the original Alpaca VHH format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | This nanobody is specific for murine CD47, a glycosylated five transmembrane protein with a small cytoplasmic domain. CD47 is involved in adhesion through interactions with SIRP (signal regulator protein) and is non-covalently associated with β3 integrins |
| Application Notes: | This nanobody has been shown to bind with high affinity and specificity to mouse CD47, as characterized by SPR and FACS (Sockolosky et al 2016). In vitro functional assays suggest that this nanobody potently blocks SIRPα binding to tumor cell-surface CD47, while broadly recognizing mouse hematopoietic and red blood cells without causing erythropenia (Sockolosky et al 2016). The original nanobody was reported to bind immobilized mouse CD47 with a binding affinity of Kd= ∼12 pM (Sockolosky et al 2016). Consistent with its species-specific binding properties, this nanobody has been found not to influence human macrophage phagocytosis of human tumor cells. Furthermore, this nanobody has been used to investigate the biological consequences of antagonizing CD47 in syngeneic mouse disease models (Sockolosky et al 2016). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |