Vector Labs Ab01103-23.0 Anti-influenza [2-8C], Rabbit IgG, lambda (200 μg) Centrifuge Bundle Savings at Sycamore Life Sciences

Description

Product Class: Purified
Clone ID: 2-8C
Synonyms: influenza NP; influenza viral nucleoprotein; influenza nucleoprotein; NP; human influenza A virus; human influenza A viruses; Influenza A virus nucleoprotein (NP); Influenza A virus NP
Amount: 200 μg
Application Codes Clone: WB; IF; IHC; ELISA; virus titration/quantification; neutralisation assays
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG1
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This chimeric rabbit antibody was made using the variable domain sequences of the original Human IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement: This antibody reacts with influenza A virus nucleoprotein (NP), which is a structural protein which encapsidates the negative strand viral RNA. As well as binding ssRNA, NP is able to self associate to form large oligomeric complexes. NP is able to interact with a variety of other macromolecules of both viral and cellular origins. It binds the PB1 and PB2 subunits of the polymerase and the matrix protein M1. "NP has also been shown to interact with at least four cellular polypeptide families: nuclear import receptors of the importin class, filamentous (F) actin, the nuclear export receptor CRM1 and a DEAD box helicase BAT1/UAP56" (Portela et al, 2002).
Application Notes: This antibody was used in virus titration and microneutralization assays to aid the development of pseudotyped influenza A virus as a vaccine for the induction of heterotypic immunity (Powell et al., 2012) and to demonstrate that a single cycle influenza virus coated in H7 hemagglutinin provides heterotypic protection and neutralising antibody responses to both glycoproteins (Powell et al., 2017). This antibody might be useful for Western blotting, immunofluorescence (IF) and immunocytochemistry (IHC), for instance, to better understand the differential replication properties among H9N2 avian influenza viruses of Eurasian origin (Parvin et al., 2015) and to validate that a conserved influenza A virus nucleoprotein code controls specific viral genome packaging (Moreira et al., 2016).

Product Class:	Purified
Clone ID:	2-8C
Synonyms:	influenza NP; influenza viral nucleoprotein; influenza nucleoprotein; NP; human influenza A virus; human influenza A viruses; Influenza A virus nucleoprotein (NP); Influenza A virus NP
Amount:	200 μg
Application Codes Clone:	WB; IF; IHC; ELISA; virus titration/quantification; neutralisation assays
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG1
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This chimeric rabbit antibody was made using the variable domain sequences of the original Human IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:	This antibody reacts with influenza A virus nucleoprotein (NP), which is a structural protein which encapsidates the negative strand viral RNA. As well as binding ssRNA, NP is able to self associate to form large oligomeric complexes. NP is able to interact with a variety of other macromolecules of both viral and cellular origins. It binds the PB1 and PB2 subunits of the polymerase and the matrix protein M1. "NP has also been shown to interact with at least four cellular polypeptide families: nuclear import receptors of the importin class, filamentous (F) actin, the nuclear export receptor CRM1 and a DEAD box helicase BAT1/UAP56" (Portela et al, 2002).
Application Notes:	This antibody was used in virus titration and microneutralization assays to aid the development of pseudotyped influenza A virus as a vaccine for the induction of heterotypic immunity (Powell et al., 2012) and to demonstrate that a single cycle influenza virus coated in H7 hemagglutinin provides heterotypic protection and neutralising antibody responses to both glycoproteins (Powell et al., 2017). This antibody might be useful for Western blotting, immunofluorescence (IF) and immunocytochemistry (IHC), for instance, to better understand the differential replication properties among H9N2 avian influenza viruses of Eurasian origin (Parvin et al., 2015) and to validate that a conserved influenza A virus nucleoprotein code controls specific viral genome packaging (Moreira et al., 2016).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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