Vector Labs Ab01105-48.1 Anti-influenza [2-12C], Ferret IgG1, (200 μg) Centrifuge Bundle Savings at Sycamore Life Sciences

Description

Product Class: Purified
Clone ID: 2-12C
Synonyms: H1N1; Influenza H1N1; Influenza A virus hemagglutinin glycoprotein; Influenza A virus HA; HA; hemagglutinin
Amount: 200 μg
Application Codes Clone: ELISA; IF; virus titration; hemagglutination inhibition test; microneutralization assays
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG1
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This chimeric ferret antibody was made using the variable domain sequences of the original Human format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement: This antibody is specific for H1N1pdm09 hemagglutinin glycoprotein (HA). Influenza A virus has one of 16 possible hemagglutinin (HA) surface proteins and one of 9 possible neuraminidase (NA) surface proteins. The hemagglutinin protein facilitates viral attachment while Neuraminidase is involved in viral release. These proteins also elicit immune responses that prevent infection or independently reduce viral replication. The pandemic H1N1pdm09 virus (other nomenclatures: H1N1/09 virus, A(H1N1)pdm09 virus, A/California/07/2009(H1N1)pdm virus, etc.) is a swine origin Influenza A virus subtype H1N1 virus strain responsible for the 2009 flu pandemic.
Application Notes: This 2-12C antibody was isolated from a donor that was exposed to A/California/07/2009 virus. It was used as a control antibody alongside with other mAbs in enzyme-linked immunosorbent, indirect immunofluorescent, virus titration, hemagglutination inhibition (HAI), and microneutralization (MN) assays for the assessment of the binding activity of HA head-specific and head cross-reactive antibodies on cells infected with the variant viruses, and for the subsequent mapping of the HA1 K163 residue in former H1N1 viruses (Huang et al., 2015). This 2-12C antibody was reported to select a variant virus with HA substitutions K130E and G170R and, to retain the capacity to neutralize clade 6B viruses at <10 ng/ml similar to the ferret antisera (Huang et al., 2015).

Product Class:	Purified
Clone ID:	2-12C
Synonyms:	H1N1; Influenza H1N1; Influenza A virus hemagglutinin glycoprotein; Influenza A virus HA; HA; hemagglutinin
Amount:	200 μg
Application Codes Clone:	ELISA; IF; virus titration; hemagglutination inhibition test; microneutralization assays
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG1
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This chimeric ferret antibody was made using the variable domain sequences of the original Human format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:	This antibody is specific for H1N1pdm09 hemagglutinin glycoprotein (HA). Influenza A virus has one of 16 possible hemagglutinin (HA) surface proteins and one of 9 possible neuraminidase (NA) surface proteins. The hemagglutinin protein facilitates viral attachment while Neuraminidase is involved in viral release. These proteins also elicit immune responses that prevent infection or independently reduce viral replication. The pandemic H1N1pdm09 virus (other nomenclatures: H1N1/09 virus, A(H1N1)pdm09 virus, A/California/07/2009(H1N1)pdm virus, etc.) is a swine origin Influenza A virus subtype H1N1 virus strain responsible for the 2009 flu pandemic.
Application Notes:	This 2-12C antibody was isolated from a donor that was exposed to A/California/07/2009 virus. It was used as a control antibody alongside with other mAbs in enzyme-linked immunosorbent, indirect immunofluorescent, virus titration, hemagglutination inhibition (HAI), and microneutralization (MN) assays for the assessment of the binding activity of HA head-specific and head cross-reactive antibodies on cells infected with the variant viruses, and for the subsequent mapping of the HA1 K163 residue in former H1N1 viruses (Huang et al., 2015). This 2-12C antibody was reported to select a variant virus with HA substitutions K130E and G170R and, to retain the capacity to neutralize clade 6B viruses at <10 ng/ml similar to the ferret antisera (Huang et al., 2015).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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