Vector Labs Ab01115-1.32-BS Anti-IgE [R1E4], Mouse IgG1-D265A, Fc Silenced, kappa (1 mg) Centrifuge Bundle Savings at Sycamore Life Sciences

Description

Product Class: Purified
Clone ID: R1E4
Heavy Chain Modification: Fc Silenced
Synonyms: Immunoglobulin E
Amount: 1 mg
Application Codes Clone: FC; ELISA
Shipping Temperature: Wet Ice
Buffer Composition: PBS only.
Original Format: IgG1
Storage Temperature: Store at 4⁰C for up to 3 months. Note, this antibody is provided without added preservatives, it is therefore recommed this antibody be handled under sterile conditions. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This chimeric mouse antibody was made using the variable domain sequences of the original Rat IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement: This antibody is specific for murine IgE. It does not react with other classes of mouse immunoglobulin including IgD, IgG, IgA, or IgM. IgE plays an essential role in type I hypersensitivity (allergic responses such as hay fever, asthma, hives, anaphylatic shock) by binding to Fc receptors on basophils and mast cells. Crosslinking of IgE bound to Fc receptors induces degranulation of mast cells and basophils that promote allergic manifestations. IgE is also functionally important for immunity against parasites.
Application Notes: In the original study, this antibody and other clones (C12B9, 23G3, and B1E3) of the monoclonal anti-mIgE antibodies were used in conjunction with the recombinant chimeric mIgE-human IgG1 molecules (Keegan et al., 1991). It has been demonstrated that the Cϵ3 domain is important in the binding of mIgE to the murine B cell FcϵRII as well as to the murine mast cell FcϵRI (Keegan et al., 1991). Also, while the Cϵ4 domain had no effect on binding to the FcϵRI, the presence of the Cϵ4 domain influenced the binding of the recombinant IgE to the FcϵRII (Keegan et al., 1991). This antibody blocks IgE binding to mouse or rat FceRI and can be used in mouse IgE ELISA. In addition, this antibody could be used, for instance, to develop a flow cytometry assay for the identification and differentiation of chemicals with the potential to elicit irritation, IgE-mediated, or T cell-mediated hypersensitivity responses (Manetz et al, 1999), or to study the kinetics of the ligand-IgE interaction (Posner et al., 1992).

Product Class:	Purified
Clone ID:	R1E4
Heavy Chain Modification:	Fc Silenced
Synonyms:	Immunoglobulin E
Amount:	1 mg
Application Codes Clone:	FC; ELISA
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS only.
Original Format:	IgG1
Storage Temperature:	Store at 4⁰C for up to 3 months. Note, this antibody is provided without added preservatives, it is therefore recommed this antibody be handled under sterile conditions. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This chimeric mouse antibody was made using the variable domain sequences of the original Rat IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:	This antibody is specific for murine IgE. It does not react with other classes of mouse immunoglobulin including IgD, IgG, IgA, or IgM. IgE plays an essential role in type I hypersensitivity (allergic responses such as hay fever, asthma, hives, anaphylatic shock) by binding to Fc receptors on basophils and mast cells. Crosslinking of IgE bound to Fc receptors induces degranulation of mast cells and basophils that promote allergic manifestations. IgE is also functionally important for immunity against parasites.
Application Notes:	In the original study, this antibody and other clones (C12B9, 23G3, and B1E3) of the monoclonal anti-mIgE antibodies were used in conjunction with the recombinant chimeric mIgE-human IgG1 molecules (Keegan et al., 1991). It has been demonstrated that the Cϵ3 domain is important in the binding of mIgE to the murine B cell FcϵRII as well as to the murine mast cell FcϵRI (Keegan et al., 1991). Also, while the Cϵ4 domain had no effect on binding to the FcϵRI, the presence of the Cϵ4 domain influenced the binding of the recombinant IgE to the FcϵRII (Keegan et al., 1991). This antibody blocks IgE binding to mouse or rat FceRI and can be used in mouse IgE ELISA. In addition, this antibody could be used, for instance, to develop a flow cytometry assay for the identification and differentiation of chemicals with the potential to elicit irritation, IgE-mediated, or T cell-mediated hypersensitivity responses (Manetz et al, 1999), or to study the kinetics of the ligand-IgE interaction (Posner et al., 1992).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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