Vector Labs Ab01119-1.32-BS Anti-GABA-A Receptor, Gamma 2 Subunit [10F10-C1-B8], Mouse IgG1-D265A, Fc Silenced, kappa (1 mg) Centrifuge Bundle Savings at Sycamore Life Sciences

Description

Product Class: Purified
Clone ID: 10F10-C1-B8
Heavy Chain Modification: Fc Silenced
Synonyms: GABARG2; GABAR-G2 CAE2; ECA2; GABA(A) receptor subunit gamma-2; GABA(A) receptor, gamma 2; gamma 2; gamma-aminobutyric acid (GABA) A receptor, gamma 2; Gamma-aminobutyric acid receptor subunit gamma-2; GEFSP3; 10F10C1B8; 10F10.C1.B8
Amount: 1 mg
Application Codes Clone: WB; IF; IHC; IP
Shipping Temperature: Wet Ice
Buffer Composition: PBS only.
Original Format: IgG2b
Storage Temperature: Store at 4⁰C for up to 3 months. Note, this antibody is provided without added preservatives, it is therefore recommed this antibody be handled under sterile conditions. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This reformatted mouse antibody was made using the variable domain sequences of the original Mouse IgG2b format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement: This antibody was generated against amino acids 37-53 of rat GABA-A receptor gamma2 subunit (GABRG2) and is specific for the N-terminal extracellular domain, allowing for the detection of surface expressed receptors for live imaging. GABA-A receptors are ligand-gated neurotransmitter receptors that function to mediate the fast synaptic inhibition within the brain. They are pentamers, composed from a variety of classes of subunits with gamma being just one class. Each subunit is composed of an extracellular N-terminal domain, four membrane spanning units, and a small extracellular C-terminal segment. Several mutations in the GABA(A) receptor subunit gamma-2 expression have been linked to epileptic syndromes that result in fibrile seizures.
Application Notes: In the original study, this antibody was used in Western Blot assays, immunohistochemistry (IHC) and immunoprecipitate (IP) for identification of the γ2 subunit from rat and mouse hippocampal proteins (Joshi et al., 2011). This antibody could also be used in functional studies, for example, to show that the GABA-A receptor membrane insertion rates are specified by their subunit composition (Joshi et al, 2013), to delineate how chorein deficiency leads to upregulation of gephyrin and GABAA receptor (Kurano et al., 2006), to examine the homeostatic strengthening of inhibitory synapses as mediated by the accumulation of GABAA receptors (Rannals et al., 2011), and to demonstrate that NMDA receptor activation downregulates expression of δ Subunit-containing GABA-A receptors in cultured hippocampal neurons (Joshi et al. 2013).

Product Class:	Purified
Clone ID:	10F10-C1-B8
Heavy Chain Modification:	Fc Silenced
Synonyms:	GABARG2; GABAR-G2 CAE2; ECA2; GABA(A) receptor subunit gamma-2; GABA(A) receptor, gamma 2; gamma 2; gamma-aminobutyric acid (GABA) A receptor, gamma 2; Gamma-aminobutyric acid receptor subunit gamma-2; GEFSP3; 10F10C1B8; 10F10.C1.B8
Amount:	1 mg
Application Codes Clone:	WB; IF; IHC; IP
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS only.
Original Format:	IgG2b
Storage Temperature:	Store at 4⁰C for up to 3 months. Note, this antibody is provided without added preservatives, it is therefore recommed this antibody be handled under sterile conditions. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This reformatted mouse antibody was made using the variable domain sequences of the original Mouse IgG2b format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:	This antibody was generated against amino acids 37-53 of rat GABA-A receptor gamma2 subunit (GABRG2) and is specific for the N-terminal extracellular domain, allowing for the detection of surface expressed receptors for live imaging. GABA-A receptors are ligand-gated neurotransmitter receptors that function to mediate the fast synaptic inhibition within the brain. They are pentamers, composed from a variety of classes of subunits with gamma being just one class. Each subunit is composed of an extracellular N-terminal domain, four membrane spanning units, and a small extracellular C-terminal segment. Several mutations in the GABA(A) receptor subunit gamma-2 expression have been linked to epileptic syndromes that result in fibrile seizures.
Application Notes:	In the original study, this antibody was used in Western Blot assays, immunohistochemistry (IHC) and immunoprecipitate (IP) for identification of the γ2 subunit from rat and mouse hippocampal proteins (Joshi et al., 2011). This antibody could also be used in functional studies, for example, to show that the GABA-A receptor membrane insertion rates are specified by their subunit composition (Joshi et al, 2013), to delineate how chorein deficiency leads to upregulation of gephyrin and GABAA receptor (Kurano et al., 2006), to examine the homeostatic strengthening of inhibitory synapses as mediated by the accumulation of GABAA receptors (Rannals et al., 2011), and to demonstrate that NMDA receptor activation downregulates expression of δ Subunit-containing GABA-A receptors in cultured hippocampal neurons (Joshi et al. 2013).

Specifications

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Brand	Vector Labs
UNSPSC	12161500

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