Mfg Part Number
Ab01123-23.0
Vector Labs Ab01123-23.0 Anti-TNFalpha [2TY], Rabbit IgG, kappa (200 μg)
Quick Overview
Affinity selection procedures were used to isolate this scFv from a naïve Tomlinson I phage display library that specifically binds to TNF-α. The panning procedure was carried out by incubating immunotubes coated with recombinant TNF-α protein with a phage suspension. Phage particles with affinity for recombinant TNF-α were amplified in TG-1 bacteria, and three rounds of panning were performed to select high affinity and specificity scFvs.
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| Product Class: | Purified |
|---|---|
| Clone ID: | 2TY |
| Synonyms: | TNFSF2; tumor necrosis factor alpha; TNF; C1K3N5; TNFA; Cachectin; TNF-alpha; TNF-a; Tumor necrosis factor ligand superfamily member 2; TNF-α |
| Amount: | 200 μg |
| Application Codes Clone: | ELISA |
| Shipping Temperature: | Wet Ice |
| Origin Pub PMID: | 29881431 |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | scFv |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This full-length, chimeric rabbit antibody was made using the variable domain sequences of the original Human scFv format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | This scFv is specific for human TNF-α. |
| Application Notes: | The binding affinity of this scFv for human TNF-α has been confirmed in ELISA analysis (Safarpour et al, 2018). This scFv could have future therapeutic applications as a TNF inhibitor. TNF inhibition is a strategy currently showing promise in the treatment of a range of inflammatory diseases, including rheumatoid arthritis and Chron's disease. |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |