Mfg Part Number
Ab01133-23.0

Vector Labs Ab01133-23.0 Anti-CD20 [1F5], Rabbit IgG, kappa (200 μg)

Quick Overview
This antibody was raised by hyperimmunising BALB/c mice with the Raji Burkett's lymphoma line and the NALM-6 pre B leukaemia line.
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Product Class:Purified
Clone ID:1F5
Synonyms:MS4A1; B-lymphocyte antigen CD20; B-lymphocyte surface antigen B1; Bp35; Leukocyte surface antigen Leu-16; Membrane-spanning 4-domains subfamily A member 1; E9PPL6; P19437
Amount:200 μg
Application Codes Clone:stimulatory; therapeutic; depleting
Shipping Temperature:Wet Ice
Buffer Composition:PBS with 0.02% Proclin 300.
Original Format:IgG2a
Storage Temperature:Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:This chimeric rabbit antibody was made using the variable domain sequences of the original mouse IgG2a format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:This antibody is specific for CD20, which is expressed by normal and malignant B cells.
Application Notes:This antibody, either alone or in combination with T cell derived factors, induces B cell proliferation via CD20 cross-linking (Clark et al, 1985).When administered to patients with refractory malignant B cell lymphomas, this antibody depletes circulating and lymph node tumour cells, with minimal toxicity (Press et al, 1987). A murine/human chimeric IgG1 construct of this antibody has been shown to lyse Burkitt's lymphoma (5RAJI) cells (Stockmeyer et al, 2000), while IgG1 and IgA2 isotypes of this antibody have been shown to kill B cells in vitro and in deplete B cells in vivo (Lohse et al, 2018). The humanized version of this antibody can be provided upon request.
More Information
Brand Vector Labs
UNSPSC 12161500