Mfg Part Number
Ab01142-10.3

Vector Labs Ab01142-10.3 Anti-HLA allele DQB1*0601 [LG11], Human IgG1, Fc Silent™, kappa (200 μg)

Quick Overview
This antibody was raised by immunising BALB/c mice with unconjugated free synthetic DQB1 peptides (region 70–90).
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Product Class:Purified
Clone ID:LG11
Heavy Chain Modification:Fc Silent™
Amount:200 μg
Application Codes Clone:ELISA; blocking
Shipping Temperature:Wet Ice
Origin Pub PMID:29034433
Buffer Composition:PBS with 0.02% Proclin 300.
Original Format:IgG1
Storage Temperature:Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:This antibody recognises peptides corresponding to the tip of the MHC antigen-binding cavity (region 70 - 90) of HLA allele DQB1*0601 (RTRAELDTVCRHNYEVAFRGI). It cross-reacts with peptide DQB1*03/70–90, and the shorter peptide DQB1*06/70–79
Application Notes:This antibody blocks the myasthenia gravis (MG)-associated DQ beta 1 allele, and is able to inhibit the proliferation of T cells from MG patients (Ayyar & Atassi, 2017). A humanised scFv derived from this antibody, which has been confirmed in ELISA analysis to recognise the same epitope, is also able to block the proliferation of T cells from MG patients typed DQB1*0601 (Ayyar & Atassi, 2017).
More Information
Brand Vector Labs
UNSPSC 12161500