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Mfg Part Number

Ab01143-2.0

Vector Labs Ab01143-2.0 Anti-PrPSc [6H10], Mouse IgG2a, kappa (100 μg)

VECTOR LABS

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Quick Overview

This antibody was raised by immunising PrP-ablated (Prnp-/-) mice with PrPSc purified from the brains of mice infected with the Obihiro prion strain.

$570.70

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Description

Product Class: Purified
Clone ID: 6H10
Synonyms: Prion; prion protein; PrP; CD230; PRNP; PRP; SIP
Amount: 100 μg
Application Codes Clone: ELISA; IP; neutralising
Shipping Temperature: Wet Ice
Origin Pub PMID: 26937029
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG2b
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This reformatted mouse antibody was made using the variable domain sequences of the original Mouse IgG2b format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement: This antibody is specific for PrPSc, and binds to a conformational epitope that incudes the extreme C-terminus of PrP. It does not react with recombinant mouse PrP or denatured PrPSc.
Application Notes: The binding specificity of this antibody for PrPSc has been confirmed in ELISA analysis; this antibody reacts with non-denatured PrPSc, but not with recombinant mouse PrP or denatured PrPSc treated with more than 3 M GdnHCl (Horiuchi et al, 2009). This antibody has been shown to immunoprecipitate PrPSc from the brains of mice, sheep, and cattle infected with prions (Horiuchi et al, 2009). Pretreatment of purified PrPSc with this antibody results in a 1 log reduction in infectious titre (Horiuchi et al, 2009).This antibody has also been used to identify C-terminal conformational differences between prion strains (Saijo et al, 2016).As this antibody recognises a conformational epitope on PrPSc, it does not react with PrPSc in immunoblot analysis (Horiuchi et al, 2009).

Product Class:	Purified
Clone ID:	6H10
Synonyms:	Prion; prion protein; PrP; CD230; PRNP; PRP; SIP
Amount:	100 μg
Application Codes Clone:	ELISA; IP; neutralising
Shipping Temperature:	Wet Ice
Origin Pub PMID:	26937029
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG2b
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This reformatted mouse antibody was made using the variable domain sequences of the original Mouse IgG2b format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:	This antibody is specific for PrPSc, and binds to a conformational epitope that incudes the extreme C-terminus of PrP. It does not react with recombinant mouse PrP or denatured PrPSc.
Application Notes:	The binding specificity of this antibody for PrPSc has been confirmed in ELISA analysis; this antibody reacts with non-denatured PrPSc, but not with recombinant mouse PrP or denatured PrPSc treated with more than 3 M GdnHCl (Horiuchi et al, 2009). This antibody has been shown to immunoprecipitate PrPSc from the brains of mice, sheep, and cattle infected with prions (Horiuchi et al, 2009). Pretreatment of purified PrPSc with this antibody results in a 1 log reduction in infectious titre (Horiuchi et al, 2009).This antibody has also been used to identify C-terminal conformational differences between prion strains (Saijo et al, 2016).As this antibody recognises a conformational epitope on PrPSc, it does not react with PrPSc in immunoblot analysis (Horiuchi et al, 2009).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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