Mfg Part Number
Ab01158-23.0
Vector Labs Ab01158-23.0 Anti-IgE [mAbA9], Rabbit IgG, kappa (200 μg)
Quick Overview
This antibody was raised by immunising ΔM1M2 BALB/c mice, which cannot express membrane-bound IgE due to the lack of the IgE-transmembrane and IgE-cytoplasmic domains, with the extracellular membrane-proximal domain of mouse membrane IgE (EMPD) conjugated
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| Product Class: | Purified |
|---|---|
| Clone ID: | mAbA9 |
| Synonyms: | mIgE; mouse Immunoglobulin E |
| Amount: | 200 μg |
| Application Codes Clone: | ELISA; FC; blocking |
| Shipping Temperature: | Wet Ice |
| Origin Pub PMID: | 18390733 |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG1 |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | This antibody is specific for the extracellular membrane-proximal domain (EMPD) of murine membrane-bound IgE. As this 19-amino acid long EMPD is isotype-specific, this antibody does not cross-react with soluble mouse IgE. |
| Application Notes: | This antibody binds to the extracellular membrane-proximal domain (EMPD) of mouse membrane-bound IgE with high specificity and affinity, as confirmed in ELISA analysis and FC analysis (Feichtner et al, 2008). In mice immunised with recombinant Bet v1a, the major birch pollen allergen, subsequent passive immunization with this antibody leads to a block of newly synthesized specific IgEs, and reduces serum Bet v1a-specific IgE titers (Feichtner et al, 2008). In vitro, this antibody induces receptor-mediated apoptosis in B cells expressing membrane-bound IgE (Feichtner et al, 2008). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |