Mfg Part Number
Ab01161-21.0-BS
Vector Labs Ab01161-21.0-BS Anti-α-gal [B1H12], Mouse IgM, kappa (1 mg)
Quick Overview
This antibody was raised by immunising α1,3galactosyltransferase knock-out mice, which lack α-gal epitopes. Mice were immunised with rabbit red cell membranes, on which α-gal is the major carbohydrate epitope.
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| Product Class: | Purified |
|---|---|
| Clone ID: | B1H12 |
| Synonyms: | alpha gal; Galα1–3Galβ1–4GlcNAc-R; alpha gal; gal |
| Amount: | 1 mg |
| Application Codes Clone: | ELISA; lysis |
| Shipping Temperature: | Wet Ice |
| Origin Pub PMID: | 11090880 |
| Buffer Composition: | PBS only. |
| Original Format: | IgM |
| Storage Temperature: | Store at 4⁰C for up to 3 months. Note, this antibody is provided without added preservatives, it is therefore recommed this antibody be handled under sterile conditions. For longer storage, aliquot and store at -20⁰C. |
| Specificity Statement: | This antibody is specific for α-gal epitopes (Galα1–3Galβ1–4GlcNAc-R) on glycoprotein and glycolipid molecules. |
| Application Notes: | This antibody binds to glycoprotein and glycolipid molecules expressing α-gal epitopes, including α-gal-BSA, in ELISA analysis (Chen et al., 2005). It does not cross-react with any glycoproteins lacking this epitope. This antibody binds to the α-gal epitopes on the hybridoma cells producing the antibody, so can mediate complement mediated autolysis in the presence of rabbit serum (Chen et al., 2000). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |