Mfg Part Number
Ab01172-2.3
Vector Labs Ab01172-2.3 Anti-4-1BB [3H3], Mouse IgG2a, Fc Silent™, kappa (200 μg)
Quick Overview
This antibody was raised by immunizing Lewis rats with 4-1BB fusion proteins, composed of the extracellular domain of murine 4-1BB and the COOH region of human IgG1 .
$517.40
Order will ship within 1-3 business days
| Product Class: | Purified |
|---|---|
| Clone ID: | 3H3 |
| Heavy Chain Modification: | Fc Silent™ |
| Synonyms: | CD137; 4 1BB; 41BB; Tumor necrosis factor receptor superfamily member 9; Tnfrsf9; T-cell antigen 4-1BB; Ila, Ly63; 4-1BB receptor |
| Amount: | 200 μg |
| Application Codes Clone: | ELISA; IP; stimulatory |
| Shipping Temperature: | Wet Ice |
| Origin Pub PMID: | 26852694 |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG2a |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This chimeric mouse antibody was made using the variable domain sequences of the original Rat IgG2a format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | This antibody is specific for murine 4-1BB, an inducible type I membrane protein that is rapidly expressed on the surface of CD4+ and CD8+ T cells after activation. |
| Application Notes: | The binding specificity of this antibody for 4-1BB has been confirmed in ELISA analysis using purified 4-1BB–Ig fusion protein, and immunoprecipitation analysis (Shuford et al, 1997).This antibody is able to block 4-1BBL binding to activated DO.11.10 T cells (Shuford et al, 1997). This antibody reacts with activated T cells, and can costimulate T cell proliferation induced by the 145.2C11 hamster anti– mouse CD3 mAb (Shuford et al, 1997). Equally, when administered to mice, this antibody expands innate immune cells, as well as host T cells (Lin et al, 2010). Administration of this antibody alone induced complete tumor regression in E.G7 tumor-bearing mice (Lin et al, 2010). Binding of this antibody to activated T cells in mice receiving heart allografts led to the rapid rejection of the allograft (Shuford et al, 1997). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |