Mfg Part Number
Ab01173-23.0
Vector Labs Ab01173-23.0 Anti-porcine VCAM [3F4], Rabbit IgG, kappa (200 μg)
Quick Overview
This antibody was raised by immunising BALB/c mice with a recombinant secreted form of porcine VCAM.
$517.40
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| Product Class: | Purified |
|---|---|
| Clone ID: | 3F4 |
| Synonyms: | pVCAM |
| Amount: | 200 μg |
| Application Codes Clone: | ELISA; blocking; FC |
| Shipping Temperature: | Wet Ice |
| Origin Pub PMID: | 9307060 |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | This antibody is specific for an epitope within domain 2 of porcine VCAM, which is expressed on activated endothelial cells. It des not cross-react with human VCAM. |
| Application Notes: | The ability of this antibody to recognise porcine VCAM has been confirmed in ELISA analysis (Mueller et al, 1995). In FC analysis, this antibody reacts with porcine VCAM on TNF-a stimulated porcine aortic endothelial cells, but not to human VCAM on TNF-a stimulated human umbilical vein endothelial cells (Mueller et al, 1995). This antibody blocks VLA-4 dependent adhesion of peripheral blood human T cells to activated porcine aortic endothelial cells (Mueller et al, 1995). It also inhibits the binding of Ramos cells to a recombinant secreted form of porcine VCAM (Mueller et al, 1995). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |