Mfg Part Number
Ab01175-23.0
Vector Labs Ab01175-23.0 Anti-Sonic Hedgehog [5E1], Rabbit IgG, kappa (200 μg)
Quick Overview
This antibody was raised by immunizing mice with purified rat SHH amino-terminal domain protein.
$517.40
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| Product Class: | Purified |
|---|---|
| Clone ID: | 5E1 |
| Synonyms: | shh; sonic hh; Q15465 |
| Amount: | 200 μg |
| Application Codes Clone: | ELISA; WB; IHC; blocking; crystallisation |
| Shipping Temperature: | Wet Ice |
| Origin Pub PMID: | 20504762 |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG1 |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | This antibody is specific for the amino-terminal domain of rat SHH (SHH-N). It cross-reacts with the two other mammalian hedgehog ligands, Indian Hedgehog and Desert Hedgehog. |
| Application Notes: | The binding specificity of this antibody for the amino-terminal fragment of SHH (SHH-N) has been confirmed in ELISA and Western blot analysis (Ericson et al, 1996).This antibody has been used to detect SHH in IHC analysis (Ericson et al, 1996).This antibody is able to inhibit hedgehog signalling, as it blocks the binding of all three mammalian Hh ligands to Ptc1 with low nanomolar affinity (Bosanac et al, 2009). In vivo, this inhibition is responsible for the observed growth retardation of HT55 an HT-29 xenografts in mice tumour models (Yauch et al, 2008). A Fab fragment derived from this antibody has been used in X ray crystallography analysis, in complex with human SHH (Maun, 2010). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |