Mfg Part Number
Ab01182-10.0
Vector Labs Ab01182-10.0 Anti-IdeS-cleaved hinge of IgG1 [C2095], Human IgG1, kappa (200 μg)
Quick Overview
This antibody was raised by immunising rabbit with a KLH‐conjugated peptide (CTAPPAPAPELLG).
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| Product Class: | Purified |
|---|---|
| Clone ID: | C2095 |
| Synonyms: | IdeS-cleaved hinge of IgG4 |
| Amount: | 200 μg |
| Application Codes Clone: | ELISA; ADCC assays; CDC assays; depletion |
| Shipping Temperature: | Wet Ice |
| Origin Pub PMID: | 24638881 |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This chimeric human antibody was made using the variable domain sequences of the original Rabbit IgG format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | C2095 targets the IdeS‐cleaved hinge of human IgG1, but not intact IgG1 or IgG1 cleaved by MMP-3 or GluV8. The antibody can also bind IdeS-cleaved hinge of human IgG4 but not IdeS-cleaved hinge of IgG2. |
| Application Notes: | ELISA demonstrated C2095 has robust binding to IdeS-generated F(ab')2 fragments of human IgG1 and human IgG4 but does not bind IdeS-generated F(ab')2 fragments of human IgG2. It also does not bind the intact IgG1, IgG2 or IgG4 counterparts (Brezski et al, 2014). ADCC and CDC assays showed C2095 is capable of restoring antibody-dependent cell-mediated cytotoxicity and complement-dependent cytotoxicity against WIL2-S cells to the otherwise inactive anti-CD20 IgG1 F(ab’)2 fragment. It also reinstated ADCC against MDA-MB-231 cells to an anti-CD142 IgG1 F(ab’)2 fragment. This antibody was also capable of eliciting both CDC and ADCC to IgG4 F(ab’)2 fragments (Brezski et al, 2014). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |