Mfg Part Number
Ab01194-1.1

Vector Labs Ab01194-1.1 Anti-Apo E [1D7], Mouse IgG1, kappa (200 μg)

Quick Overview
BALB/c mice were used, were injected twice with 50 ug of purified apo E 1 week apart.
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Product Class:Purified
Clone ID:1D7
Synonyms:Human apolipoprotein E
Amount:200 μg
Application Codes Clone:RIA; SPR; ELISA
Shipping Temperature:Wet Ice
Origin Pub PMID:8558079
Buffer Composition:PBS with 0.02% Proclin 300.
Original Format:IgG1
Storage Temperature:Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Specificity Statement:Is specific for the human apolipoprotein E antigen, and appears to be sensitive to lysine modifications and binds very poorly to carbamylated Apo E. The epitope corresponds to the LDLr binding site on Apo E, and incorporation into lipid vesicles does not affect its immunoreactivity.
Application Notes:The binding specificity of this antibody has been confirmed using a radioimmunoassay showing it to be specific to Apo E using a radioimmunoassay which is sensitive to carbamylation and methlyation (Milne et al, 1981). Surface plasmon resonance has been used to analyse the kinetics of the binding of 1D7 (Raffai et al, 1995). Sandwich assays have also been used to determine the epitope 1D7 binds to.
More Information
Brand Vector Labs
UNSPSC 12161500