Mfg Part Number
Ab01197-10.0
Vector Labs Ab01197-10.0 Anti-EPCAM [B38.1], Human IgG1, kappa (200 μg)
Quick Overview
Mice were immunized with membrane-enriched fractions of human metastic mammary carcinoma cells. The spleens were extracted and fused with NS-1 to generate primary hybridoma cultures. These were screened using solid phase RIA for those which were reactive with metastic mammary tumor cells from livers but were not reactive with healthy livers.
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| Product Class: | Purified |
|---|---|
| Clone ID: | B38.1 |
| Synonyms: | DIAR5; epithelial cell adhesion molecule; ESA; KSA; M4S1; MK-1; EGP-2; ING-1 |
| Amount: | 200 μg |
| Application Codes Clone: | RIA; ELISA |
| Shipping Temperature: | Wet Ice |
| Origin Pub PMID: | 1873500 |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG1 |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | Reactive against 80% of primary mammary tumors. Has a binding constant in the nM range. |
| Application Notes: | Has been used to generate a human engineered monoclonal antibody (Ammons et al, 2003). B38.1 has been shown to be able to mediate antibody-dependent cytotoxicity (Robinson et al, 1991). It has undergone clinical trials as an anti-carcinoma drug (de Bono et al, 2002 and Better et al, 2002). It has also been used in ELISAs to test the reactivity of its humanised construct, ING-1, that it has an affinity in the nM range by competition assays, and it has been shown to be active in vivo (Ammons et al, 2003). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |