Mfg Part Number
Ab01198-34.11
Vector Labs Ab01198-34.11 Anti-FedF [NbFedF9], Camelid VHH, His-tagged, (100 μg)
Quick Overview
A Llama was immunised 6 times with purified FedF residues 15-165 over a period of 6 weeks. Lymphocytes were extracted and used to prepare cDNA which was used as a template fthe VHH. These were then transfected into E. coli and the antibodies selected by phage display. This was then subjected to ELISA to confirm the specificity of the antibodies.
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| Product Class: | Purified |
|---|---|
| Clone ID: | NbFedF9 |
| Heavy Chain Modification: | His-tagged |
| Synonyms: | F18 fimbrial adhesin AC; F18 fimbrial adhesin |
| Amount: | 100 μg |
| Application Codes Clone: | ELISA; MST; SPR; blocking |
| Shipping Temperature: | Wet Ice |
| Origin Pub PMID: | 25502211 |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | VHH |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Specificity Statement: | Specific to the N terminal lectin domain as this is the domain represented in the 15-165 fragment. It has an affinity for FedF(15–165) of around 1.5 nM (Moones et al, 2014). |
| Application Notes: | It has been shown that this antibody can inhibit the binding of FedF to piglent enterocytes. The structure of this antibody binding to FedF has been resolved to a resolution of 1.5A. This shows that the mechanism of inhibition of FedF binding to epithelial cell surfaces is by competing at the carbohydrate binding site. Both MST and SPR have been used to analyse binding affinities (Moones et al, 2014). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |