Mfg Part Number
Ab01199-23.0
Vector Labs Ab01199-23.0 Anti-alpha-5 TMD [Nb25], Rabbit IgG, kappa (200 μg)
Quick Overview
A llama was immunized six times with recombinant 1D4-tagged human a1b3 GABAAR pentamer. Nanobodies were selected by phage display, and the clones were then screened for binding to the receptor. From these clones Nb25 was selected.
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| Product Class: | Purified |
|---|---|
| Clone ID: | Nb25 |
| Synonyms: | GABRA5; Gamma-aminobutyric acid receptor subunit alpha-5; GABA(A) receptor subunit alpha-5 |
| Amount: | 200 μg |
| Application Codes Clone: | Crystallisation |
| Shipping Temperature: | Wet Ice |
| Origin Pub PMID: | 28991263 |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | VHH |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This chimeric rabbit antibody was made using the variable domain sequences of the original VHH format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | Binds to the transmembrane domain of the alpha-5 GABAA receptor. To see the exact epitope see the crystal structure (Miller et al, 2017). Nb25 straddles the neurotransmitter-binding site between adjacent subunits. |
| Application Notes: | The antibody was used to facilitate the crystallisation of the a5 GABAA receptor transmembrane domain where it was shown to form a 1080A2 area interface (approx.). Nb25 was used to increase the soluability and aid packing in the crystal. It has been shown that the binding of Nb25 does not affect the potentiation of the receptor. (Miller et al, 2017) |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |