Vector Labs Ab01200-10.3 Anti-Protein C [HPC-4], Human IgG1, Fc Silent™, kappa (200 μg) Centrifuge Bundle Savings at Sycamore Life Sciences

Description

Product Class: Purified
Clone ID: HPC-4
Heavy Chain Modification: Fc Silent™
Synonyms: Autoprothrombin IIA; C-tag; Protein C tag; Protein C-tag; HPC4; Vitamin K-dependent protein C; Anticoagulant protein C; Blood coagulation factor XIV; PROC
Amount: 200 μg
Application Codes Clone: Blocking; Purification; ELISA
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG1
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement: Specifically binds to a specific twelve peptide sequence (EDQVDPRLIDGK) in the activation region of the Protein C (spanning the thrombin cleavage site). Does not react with activated protein C. Has been shown to be cross-reactive with baboon Protein C but not with bovine or mouse Protein C.
Application Notes: HPC-4 binds human protein C only in the presence of Ca²⁺; it binds to the reduced carboxymethylated protein C heavy chain, and it blocks protein C activation but does not bind or inactivate protein C or activated protein C bound to its plasma inhibitor. Furthermore, it is an anti-epitope tag antibody, as it recognizes the EDQVDPRLIDGK sequence (Taylor Jr et al., 1987; 3102560). It has been shown to work in ELISAs to determine expression levels (Velander et al., 1992; PMID: 1465430). HPC-4 has been shown to be effective at purifying proteins tagged with its epitope (Rezaie et al., 1992; PMID: 1283093). It has also been used to block the activation of Protein C by the thrombin-thrombomodulin complex (Stearns et al., 1988; PMID: 2447082) and was used for the same purpose in Baboons to lower the threshold of sepsis (Taylor Jr et al., 1987; PMID: 3102560). HPC-4 has been used in many other studies to observe the function of Protein C by knocking out its function in a Ca²⁺-dependent manner, purification, and others (Tanaka et al., 2006; PMID: 16398653) (Bae et al., 2007; PMID: 17823308).

Product Class:	Purified
Clone ID:	HPC-4
Heavy Chain Modification:	Fc Silent™
Synonyms:	Autoprothrombin IIA; C-tag; Protein C tag; Protein C-tag; HPC4; Vitamin K-dependent protein C; Anticoagulant protein C; Blood coagulation factor XIV; PROC
Amount:	200 μg
Application Codes Clone:	Blocking; Purification; ELISA
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG1
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:	Specifically binds to a specific twelve peptide sequence (EDQVDPRLIDGK) in the activation region of the Protein C (spanning the thrombin cleavage site). Does not react with activated protein C. Has been shown to be cross-reactive with baboon Protein C but not with bovine or mouse Protein C.
Application Notes:	HPC-4 binds human protein C only in the presence of Ca²⁺; it binds to the reduced carboxymethylated protein C heavy chain, and it blocks protein C activation but does not bind or inactivate protein C or activated protein C bound to its plasma inhibitor. Furthermore, it is an anti-epitope tag antibody, as it recognizes the EDQVDPRLIDGK sequence (Taylor Jr et al., 1987; 3102560). It has been shown to work in ELISAs to determine expression levels (Velander et al., 1992; PMID: 1465430). HPC-4 has been shown to be effective at purifying proteins tagged with its epitope (Rezaie et al., 1992; PMID: 1283093). It has also been used to block the activation of Protein C by the thrombin-thrombomodulin complex (Stearns et al., 1988; PMID: 2447082) and was used for the same purpose in Baboons to lower the threshold of sepsis (Taylor Jr et al., 1987; PMID: 3102560). HPC-4 has been used in many other studies to observe the function of Protein C by knocking out its function in a Ca²⁺-dependent manner, purification, and others (Tanaka et al., 2006; PMID: 16398653) (Bae et al., 2007; PMID: 17823308).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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