Mfg Part Number
Ab01207-10.3
Vector Labs Ab01207-10.3 Anti-MOG [M3-24], Human IgG1, Fc Silent™, kappa (200 μg)
Quick Overview
Callithrix jacchus marmosets were used, and experimental allergic encephalomyelitis was induced by the injection of rat MOG (aa1-125) into the marmosettes. The rMOG was expressed in E. coli and purified to homogeneity. The animals were killed 4-70 days after the onset of symptoms of EAE. Bone marrow and spleen cells were obtained from an immunized C. jacchus, the RNA extracted with Trizol reagent and rtPCR used to generate cloning inserts containing Fab portions of IgGk. Phage display was then used to select for the MOG-reactive Fab fragments using the pCOMB3H phage display vector, and binding confirmed using an ELISA.
$517.40
Order will ship within 1-3 business days
| Product Class: | Purified |
|---|---|
| Clone ID: | M3-24 |
| Heavy Chain Modification: | Fc Silent™ |
| Synonyms: | Myelin oligodendrocyte glycoprotein; rMOG |
| Amount: | 200 μg |
| Application Codes Clone: | ELISA; blocking; IHC |
| Shipping Temperature: | Wet Ice |
| Origin Pub PMID: | 12060766 |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | Fab |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This chimeric human antibody was made using the variable domain sequences of the original Fab format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | Recognises an epitope which is distinct from M26, M38, M45, M3-8 and M3-31. The epitope recognised is a structural epitope, shown by the lack of recognition of linear MOG peptides. Is unique to recombinant hMOG125 and rMOG125, but is not exposed on recombinant hMOG118; hence this epitope is species-independent, but dependent on the length of the protein (Menge et al, 2011). |
| Application Notes: | This antibody has been proposed for the diagnosis and prognosis of multiple sclerosis (MS) or allergic encephalomyelitis (EAE) by using competition assays to determine if there are autoantibodies present in an individual. It has also been proposed that it could be used to predict the severity of the MS or EAE by detecting the proportion of conformation-specific autoantibodies to linear epitope autoantibodies. This antibody has been used in ELISAs and competition assays to characterise its epitope in relation to other antibodies generated against MOG. It was originally generated and tested as a Fab fragment (vod Budingen et al, 2002). Whilst this has been shown to not bind to hMOG, it has been shown to compete with IgG from MS patients for binding to rMOG (Lalive et al, 2006). Analysis has also been done on the amino acid sequence of this antibody (von Budingen et al, 2006). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |