Mfg Part Number
Ab01209-10.3
Vector Labs Ab01209-10.3 Anti-Endosialin [FB5], Human IgG1, Fc Silent™, kappa (200 μg)
Quick Overview
BALB/cxA F1 mice were immunised with human fibroblasts with subsequent production and screening of hybridoma cells.
$517.40
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| Product Class: | Purified |
|---|---|
| Clone ID: | FB5 |
| Heavy Chain Modification: | Fc Silent™ |
| Synonyms: | CD248; FB5; tumor endothelial marker 1 |
| Amount: | 200 μg |
| Application Codes Clone: | IHC; IP; WB; SFA |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG2a |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG2a format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | Endosialin is not expressed in normal human tissues. FB5 is reactive to cultured fibroblasts and neuroblastoma cell lines, and tumour vascular endothelial cells. |
| Application Notes: | FB5 was used in immunohistochemistry experiments to determine the distribution of endosialin in cultured human cells and normal adult tissues (Rettig et al, 1992). FB5 was used in immunoprecipitation and Western blotting to characterise endosialin (Rettig et al, 1992). In hemadsorption rosetting assays with LA1-5s cells the concentration of FB5 required for 50% rosetting was determined as 3 ng/ml. |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |