Mfg Part Number
Ab01209-2.3

Vector Labs Ab01209-2.3 Anti-Endosialin [FB5], Mouse IgG2a, Fc Silent™, kappa (200 μg)

Quick Overview
BALB/cxA F1 mice were immunised with human fibroblasts with subsequent production and screening of hybridoma cells.
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Product Class:Purified
Clone ID:FB5
Heavy Chain Modification:Fc Silent™
Synonyms:CD248; FB5; tumor endothelial marker 1
Amount:200 μg
Application Codes Clone:IHC; IP; WB; SFA
Shipping Temperature:Wet Ice
Buffer Composition:PBS with 0.02% Proclin 300.
Original Format:IgG2a
Storage Temperature:Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Specificity Statement:Endosialin is not expressed in normal human tissues. FB5 is reactive to cultured fibroblasts and neuroblastoma cell lines, and tumour vascular endothelial cells.
Application Notes:FB5 was used in immunohistochemistry experiments to determine the distribution of endosialin in cultured human cells and normal adult tissues (Rettig et al, 1992). FB5 was used in immunoprecipitation and Western blotting to characterise endosialin (Rettig et al, 1992). In hemadsorption rosetting assays with LA1-5s cells the concentration of FB5 required for 50% rosetting was determined as 3 ng/ml.
More Information
Brand Vector Labs
UNSPSC 12161500