Mfg Part Number
Ab01212-23.0
Vector Labs Ab01212-23.0 Anti-Feline Calicivirus [1D7], Rabbit IgG, kappa (200 μg)
Quick Overview
The original antibody was raised by immunizing BALB/c mice with feline calcivirus infected CRFK cells. After two weeks, mouse lymphocytes and mouse myeloma cells (P3U1) were cell-fused with polyethylene glycol to prepare hybridomas.
$517.40
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| Product Class: | Purified |
|---|---|
| Clone ID: | 1D7 |
| Synonyms: | FCV; Capsid protein; Coat protein; CP; VP1 |
| Amount: | 200 μg |
| Application Codes Clone: | NTRL; ELISA |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG2b |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2b format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | 1D7 is capable of neutralizing all these FCV strains: F4, F1, F3, F5, F7, F6, F8, F11, F14, F25, FC, FR, FPL, CFI, FJ, 227, 337, and KDC. Does not recognise the denatured antigen. |
| Application Notes: | This antibody binds and neutralizes the feline calicivirus. It is capable of detecting the virus in an ELISA. Neutralisation assays confirmed 1D7 neutralises feline calicivirus at 208.2 ng/ml. The Ki of 1D7 is 1 μg/ml. This antibody is not capable of recognizing the denatured FCV antigen in an immunoblot assay. It was considered that the 1D7 epitope might exist in a conserved region on the capsid protein because of the ability of 1D7 to neutralize all the strains of FCV tested (PMID:1708228). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |