Mfg Part Number
Ab01214-1.1

Vector Labs Ab01214-1.1 Anti-Rhesus D antigen [D7C2], Mouse IgG1, kappa (200 μg)

Quick Overview
A Rh- volunteer blood donor (r/r) was injected with 5ml of Rh+ erythrocytes (R1/r) and the peripheral lymphocytes were isolated and transformed with 1 ml of culture supernatant of the EBV marmoset B96-8 cell line.
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Product Class:Purified
Clone ID:D7C2
Synonyms:CD240D; RHXIII; Rh polypeptide 2; RhPII
Amount:200 μg
Application Codes Clone:FC; ADCC
Shipping Temperature:Wet Ice
Buffer Composition:PBS with 0.02% Proclin 300.
Original Format:IgM
Storage Temperature:Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:This chimeric mouse antibody was made using the variable domain sequences of the original Human format, for improved compatibility with existing reagents, assays and techniques.
Application Notes:FC confirmed the binding of D7C2 to Rh D+ red cells in artifical mixtures (Edelman et al, 1997). The ADCC of D7C2 showed a percentage of 93% lysis (Edelman et al, 1997). D7C2 has been expressed recombinantly using a baculovirus-insect cell expression system (Edelman et al, 1997).
More Information
Brand Vector Labs
UNSPSC 12161500