Mfg Part Number
Ab01215-10.0
Vector Labs Ab01215-10.0 Anti-c-erbB-2 [FWP51], Human IgG1, kappa (200 μg)
Quick Overview
20x10^6 SKBR3 human breast tumour cells were injected into BALB/c mice. The hybridomas generated from the mice spleen cells were then screen for antibodies reactive to c-erbB-2 using immunofluorescence and immunoprecipitation.
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| Product Class: | Purified |
|---|---|
| Clone ID: | FWP51 |
| Synonyms: | CD340; Receptor tyrosine-protein kinase erbB-2; Neu; HER2; MLN19; Metastatic lymph node gene 19 protein; p185erbB2 |
| Amount: | 200 μg |
| Application Codes Clone: | IF; IP; FC; fusion |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG1 |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | FWP51 binds to the extracellular domain of c-erbB-2. |
| Application Notes: | Immunofluorescence and immunoprecipitation were used to confirm the specificity of FWP51 for c-erbB-2 (Harwerth et al, 1992). Flow cytometry showed that FWP51 binds the extracellular domain of c-erbB-2 (Harwerth et al, 1992). FWP51 has also been fused to the bacterial toxin, exotoxin A. The resultant fusion protein Fv(FWP51)-ETA selectively inhibited protein synthesis in cells expressing high levels of human c-erbB-2 protein, but not in cells expressing little to no human c-erbB-2 protein. Administration of FWP51 to mice impanted with human erbB-2 transformed NIH3T3 cells led to inhibition of the onset of tumour growth (Harwerth et al, 1993). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |