Vector Labs Ab01219-23.0 Anti-CR1 [E11], Rabbit IgG, kappa (200 μg) Centrifuge Bundle Savings at Sycamore Life Sciences

Description

Product Class: Purified
Clone ID: E11
Synonyms: CD35; Type 1 complement receptor; C3b/C4b receptor
Amount: 200 μg
Application Codes Clone: ELISA; Western blot; IF
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG1
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement: E11 is reactive to neutrophilic granulocytes and eosinophils; monocytes; macrophages; lymphocytes; erythrocytes; dendritic reticulum cells; tonsil tissue section; spleen tissue section; lymph node tissue section; gut tissue section; kidney podocytes and kidney tissue sections; chronic lymphocytic leukemia; acute myelomonocytic leukemia; acute monocyte leukemia; B cell lines (JY, MST, BR17); EBV-infected B cell cultures; myeloid cell lines (U937, HL60, THP 1-0).
Application Notes: E11 fluorescence staining of normal blood cells was detectable on 90% of monocytes, 95% of neutrophils, and 14%-24% of lymphocytes (Hogg et al, 1984). Assay of E11 fluorescence-stained monocytes and neutrophils by EC3b rosette formation indicated that nearly all of the E11-stained cells formed rosettes with EC3b (Hogg et al, 1984). Treatment of erythrocytes, monocytes, neutrophils, and lymphocytes with E11 did not result in any detectable loss of CR1 activity as measured by EC3b rosette formation (Hogg et al, 1984). Immunofluorescence was used to determine E11 reactivity to a variety of cells, tissue sections, leukemias, and cell lines (Hogg et al, 1984). E11 has been used in ELISA experiments to quantify the amount of recombinant CR1 proteins (Park et al, 2013).

Product Class:	Purified
Clone ID:	E11
Synonyms:	CD35; Type 1 complement receptor; C3b/C4b receptor
Amount:	200 μg
Application Codes Clone:	ELISA; Western blot; IF
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG1
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:	E11 is reactive to neutrophilic granulocytes and eosinophils; monocytes; macrophages; lymphocytes; erythrocytes; dendritic reticulum cells; tonsil tissue section; spleen tissue section; lymph node tissue section; gut tissue section; kidney podocytes and kidney tissue sections; chronic lymphocytic leukemia; acute myelomonocytic leukemia; acute monocyte leukemia; B cell lines (JY, MST, BR17); EBV-infected B cell cultures; myeloid cell lines (U937, HL60, THP 1-0).
Application Notes:	E11 fluorescence staining of normal blood cells was detectable on 90% of monocytes, 95% of neutrophils, and 14%-24% of lymphocytes (Hogg et al, 1984). Assay of E11 fluorescence-stained monocytes and neutrophils by EC3b rosette formation indicated that nearly all of the E11-stained cells formed rosettes with EC3b (Hogg et al, 1984). Treatment of erythrocytes, monocytes, neutrophils, and lymphocytes with E11 did not result in any detectable loss of CR1 activity as measured by EC3b rosette formation (Hogg et al, 1984). Immunofluorescence was used to determine E11 reactivity to a variety of cells, tissue sections, leukemias, and cell lines (Hogg et al, 1984). E11 has been used in ELISA experiments to quantify the amount of recombinant CR1 proteins (Park et al, 2013).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

Reviews