Mfg Part Number
Ab01220-5.0
Vector Labs Ab01220-5.0 Anti-Phosphatidylinositol-3,4-diphosphate [8C2], Mouse IgG3, kappa (200 μg)
Quick Overview
Salmonella minnesota cells were coated with 10 μg of PI 3,4‐P2 and injected into mice. Hybridomas were formed by fusion with SP2/0 cells, and the hybridoma supernatants screened for antibodies against PI 3,4‐P2 by a liposome lysis assay.
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| Product Class: | Purified |
|---|---|
| Clone ID: | 8C2 |
| Synonyms: | PtdIns(3,4)P2; PI-3,4-P2; PIP2 |
| Amount: | 200 μg |
| Application Codes Clone: | Liposome lysis assay; IB; IF |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG3 |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Specificity Statement: | 8C2 does not bind PI-4,5-P2, PI-3,4-P2, or PI-4-P2 and is specific towards PI-3,4-P2. It also does not bind PC, PE, PS, PA, PI. |
| Application Notes: | Liposome lysis assays established that 8C2 does not bind to PI-4-P, PI-4,5-P2 or PIP3 molecules. This was also confirmed by a dot-blot assay. 8C2 was used to stain 293T cells for PI-3,4-P2 with a second antibody conjugated with FITC. |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |