Mfg Part Number
Ab01221-10.3

Vector Labs Ab01221-10.3 Anti-Cyclosporin A [R454511], Human IgG1, Fc Silent™, kappa (200 μg)

Quick Overview
BALB/c mice were immunised with a D-Lys8-Cs derivative coupled to a carrier protein.
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Product Class:Purified
Clone ID:R454511
Heavy Chain Modification:Fc Silent™
Synonyms:CsA; ciclosporin A, cyclosporine A
Amount:200 μg
Application Codes Clone:ELISA; crystallography
Shipping Temperature:Wet Ice
Origin Pub PMID:1566062
Buffer Composition:PBS with 0.02% Proclin 300.
Original Format:IgG1
Storage Temperature:Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:R454511 binds to residues 1, 2, 3, 4, 5, 9 and 11 of CsA.
Application Notes:Mutagenesis of Cs-derivatives identified that R454511 binds to residues 1, 2, 3, 4, 5, 9 and 11 of CsA (Quesniaux et al., 1990). In ELISA experimenets, R454511 was found to bind to Cs conjugated with both Thr2 (Thr2-Cs-BSA) and Lys8 (D-Lys8-Cs-BSA) (Quesniaux et al., 1990). The crystal structure of R454511 in complex with CsA has been obtained to 2.65 Å resolution (Altschuh et al., 1992).
More Information
Brand Vector Labs
UNSPSC 12161500