Mfg Part Number
Ab01222-23.0
Vector Labs Ab01222-23.0 Anti-HCV NS3 Protein [Hfab-aNS3], Rabbit IgG, kappa (200 μg)
Quick Overview
Hfab-aNS3 was isolated from a phage display library obtained from the IgG1/kappa repertoire of an HCV-infected patient who suffered from chronic hepatitis.
$517.40
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| Product Class: | Purified |
|---|---|
| Clone ID: | Hfab-aNS3 |
| Synonyms: | Genome polyprotein; Core protein p21; Capsid protein C; p21; Core protein p19; Envelope glycoprotein E1; gp32; gp35; Envelope glycoprotein E2; NS1; gp68; gp70; p7; Protease NS2-3; p23; Serine protease NS3; Hepacivirin; NS3P; p70; Non-structural protein 4A |
| Amount: | 200 μg |
| Application Codes Clone: | ELISA; helicase assay; ICC |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG1 |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This chimeric rabbit antibody was made using the variable domain sequences of the original Human IgG1 format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | Hfab-aNS3 binds HCV NS3 protein and is directed against a conformational epitope, as it can bind NS3 in ELISA assays but not Western blots. |
| Application Notes: | ELISA was used to demonstrate that Hfab-aNS3 binds to NS3 protein, with the relative affinity found to be in the range of 1 nmol/L (Prabhu et al, 2004). Through helicase assays it was established that Hfab-aNS3 blocks the helicase activity of NS3 protein (Prabhu et al, 2004). Intracellular expression of Hfab-aNS3 inhibits replication and expression of NS3 protein in a replicon cell line, as shown through immunocytochemistry (Prabhu et al, 2004). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |