Mfg Part Number
Ab01223-1.1
Vector Labs Ab01223-1.1 Anti-Ghrelin [1621], Mouse IgG1, kappa (200 μg)
Quick Overview
Mice were immunised with human ghrelin acylated at the His residue position 9.
$517.40
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| Product Class: | Purified |
|---|---|
| Clone ID: | 1621 |
| Synonyms: | Appetite-regulating hormone; growth hormone secretagogue; protein M46 |
| Amount: | 200 μg |
| Application Codes Clone: | ELISA; calcium assay; SPR |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | Fab |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This reformatted mouse antibody was made using the variable domain sequences of the original Mouse Fab format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | The antibody has specificity for both the acylated and unacylated form of ghrelin. |
| Application Notes: | ELISA demonstrated that 1621 binds full length human ghrelin independent of its acylation status, and binds poorly to rat ghrelin. The in vitro FLIPR Calcium Assay system was used to show that 1621 binds residues 4-20 of human ghrelin. Using SPR, the Kd of 1621 binding to acylated human ghrelin was determined as 6.80 x 10^-10 M; the Kd of 162 binding to deacylated human ghrelin was determined as 1.74 x 10^-9 M. |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |