Mfg Part Number
Ab01227-10.0
Vector Labs Ab01227-10.0 Anti-Lewis Y [3S193], Human IgG1, kappa (200 μg)
Quick Overview
3S193 (IgG3) was generated in BALB/c mice by immunisation with Ley-expressing cells of the MCF-7 breast carcinoma cell-line.
$517.40
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| Product Class: | Purified |
|---|---|
| Clone ID: | 3S193 |
| Synonyms: | Ley |
| Amount: | 200 μg |
| Application Codes Clone: | humanisation; ELISA; IHC |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG3 |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG3 format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | Lewis Y (Ley) is a blood group antigen expressed at high levels on the surface of epithelial tumors, including small cell lung cancer. |
| Application Notes: | The murine 3S193 showed high specificity for Ley in ELISA tests with synthetic Ley and Ley-containing glycoproteins and glycolipids and also reacted strongly in rosetting assays and cytotoxic tests with Ley-expressing cells (Scott et al, 2000). Murine 3S193 was humanised and in antibody-dependent cell-mediated cytotoxicity assays displayed 100-fold greater activity (Kitamura et al, 1994). 3S193 has been used to screen tumour samples for Ley expression using immunohistochemistry (Krug et al, 2007). A crystal structure of the humanised antibody has been obtained to 1.9 Å resolution (Ramsland et al, 2004). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |