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Mfg Part Number

Ab01232-7.4

Vector Labs Ab01232-7.4 Anti-Transferrin receptor [RI7 217.1.3], Rat IgG2a, Fc Silent™, kappa (200 μg)

VECTOR LABS

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Quick Overview

The antibody was obtained from a fusion of the murine myeloma S 194/5.XXO.BU. 1 with spleen cells from a BDIX rat immunized with the Friend erythroleukemia 745.6.

$517.40

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Description

Product Class: Purified
Clone ID: RI7 217.1.3
Heavy Chain Modification: Fc Silent™
Synonyms: CD71; Transferrin receptor protein 1; TR; ; TfR1; Trfr; R17 217.1.3; RI7-217
Amount: 200 μg
Application Codes Clone: FC; depletion
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG2a
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Specificity Statement: The transferrin receptor is necessary for cellular iron uptake by the process of receptor-mediated endocytosis. Mice that are deficient in this receptor show impaired erythroid development and abnormal iron homeostasis.
Application Notes: This antibody was generated and used in a flow cytometry assay to identify murine hematopoietic progenitor cells expressing the transferrin receptor (Lesley et al., 1984; PMID: 6319033). The original format of this antibody was tested and found to moderately reduce cell growth. A Fab version of this antibody was generated, its binding was tested, and it was compared to its full antibody counterpart. In contrast to the original antibody format, the Fab fragment did not cause receptor down-regulation or accelerated degradation. Both the Fab fragment and the full antibody were successfully used in an IF staining assay (Lesley et al., 1989; PMID: 2653853). The original fotmat of this antibody was demonstrated to undergo receptor-mediated transcytosis across the blood-brain barrier (BBB), as confirmed by capillary depletion studies, and to effectively transport into the mouse brain in vivo (Lee et al., 2000; PMID: 10688622). Injection of this antibody into mice resulted in CD71⁺ erythroid splenocyte cell depletion (Torow et al., 2015; PMID: 26195040).

Product Class:	Purified
Clone ID:	RI7 217.1.3
Heavy Chain Modification:	Fc Silent™
Synonyms:	CD71; Transferrin receptor protein 1; TR; ; TfR1; Trfr; R17 217.1.3; RI7-217
Amount:	200 μg
Application Codes Clone:	FC; depletion
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG2a
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Specificity Statement:	The transferrin receptor is necessary for cellular iron uptake by the process of receptor-mediated endocytosis. Mice that are deficient in this receptor show impaired erythroid development and abnormal iron homeostasis.
Application Notes:	This antibody was generated and used in a flow cytometry assay to identify murine hematopoietic progenitor cells expressing the transferrin receptor (Lesley et al., 1984; PMID: 6319033). The original format of this antibody was tested and found to moderately reduce cell growth. A Fab version of this antibody was generated, its binding was tested, and it was compared to its full antibody counterpart. In contrast to the original antibody format, the Fab fragment did not cause receptor down-regulation or accelerated degradation. Both the Fab fragment and the full antibody were successfully used in an IF staining assay (Lesley et al., 1989; PMID: 2653853). The original fotmat of this antibody was demonstrated to undergo receptor-mediated transcytosis across the blood-brain barrier (BBB), as confirmed by capillary depletion studies, and to effectively transport into the mouse brain in vivo (Lee et al., 2000; PMID: 10688622). Injection of this antibody into mice resulted in CD71⁺ erythroid splenocyte cell depletion (Torow et al., 2015; PMID: 26195040).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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