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Mfg Part Number

Ab01233-1.1

Vector Labs Ab01233-1.1 Anti-CD11a [MHM24], Mouse IgG1, kappa (200 μg)

VECTOR LABS

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Quick Overview

Spleen cells from BALB/c mice immunised with EBV-transformed human lymphoblastoid cells were fused with P3-NSUlAg4.1 myeloma cells and the resulting hybridomas were screened for antibody production by a radioimmunoassay on T and B cell lines.

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Description

Product Class: Purified
Clone ID: MHM24
Synonyms: Integrin alpha-L; CD11 antigen-like family member A; leukocyte function-associated molecule 1 alpha chain; human lymphocyte function antigen
Amount: 200 μg
Application Codes Clone: cytofluorography; inhibition studies; humanisation
Shipping Temperature: Wet Ice
Origin Pub PMID: 8943405
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This reformatted mouse antibody was made using the variable domain sequences of the original Mouse IgG format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement: MHM24 binds to the CD11a subunit of lymphocyte function-associated antigen. It binds to both the high- and low-affinity forms of the antigen.
Application Notes: Cytofluorography was used to show the binding of MHM24 to peripheral blood lymphocytes, with 97.3% of cells stained by MHM24 (Hildreth et al, 1983). In inhibition experiments, MHM24 inhibited T cell-mediated lysis of virus-infected cells (Hildreth et al, 1983). Additionally, MHM24 inhibited NK cell-mediated lysis (Hildreth et al, 1983).Flow cytometry showed MHM24 exhibits non-selective binding to high-affinity and low-affinity forms of LFA-1 (Huang et al, 2006). Murine MHM24 was humanised and shown to prevent adhesion of human T cells to human keratinocytes and the proliferation of T cells in response to nonautologous leukocytes (Werther et al, 1996).

Product Class:	Purified
Clone ID:	MHM24
Synonyms:	Integrin alpha-L; CD11 antigen-like family member A; leukocyte function-associated molecule 1 alpha chain; human lymphocyte function antigen
Amount:	200 μg
Application Codes Clone:	cytofluorography; inhibition studies; humanisation
Shipping Temperature:	Wet Ice
Origin Pub PMID:	8943405
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This reformatted mouse antibody was made using the variable domain sequences of the original Mouse IgG format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:	MHM24 binds to the CD11a subunit of lymphocyte function-associated antigen. It binds to both the high- and low-affinity forms of the antigen.
Application Notes:	Cytofluorography was used to show the binding of MHM24 to peripheral blood lymphocytes, with 97.3% of cells stained by MHM24 (Hildreth et al, 1983). In inhibition experiments, MHM24 inhibited T cell-mediated lysis of virus-infected cells (Hildreth et al, 1983). Additionally, MHM24 inhibited NK cell-mediated lysis (Hildreth et al, 1983).Flow cytometry showed MHM24 exhibits non-selective binding to high-affinity and low-affinity forms of LFA-1 (Huang et al, 2006). Murine MHM24 was humanised and shown to prevent adhesion of human T cells to human keratinocytes and the proliferation of T cells in response to nonautologous leukocytes (Werther et al, 1996).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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