Mfg Part Number
Ab01234-23.9
Vector Labs Ab01234-23.9 Anti-Complement C3 [KF7], Rabbit IgG-Fc fusion, His-Tagged, (200 μg)
Quick Overview
A female New Zealand White rabbit was immunised with guinea pig C3 by three subsequent subcutaneous injections. The RNA was isolated from bone marrow, a phage display library constructed and then used to produce soluble scFv fragments.
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| Product Class: | Purified |
|---|---|
| Clone ID: | KF7 |
| Heavy Chain Modification: | His-Tagged |
| Synonyms: | C3 |
| Amount: | 200 μg |
| Application Codes Clone: | ELISA; SPR |
| Shipping Temperature: | Wet Ice |
| Origin Pub PMID: | 10699585 |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | scFv |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This chimeric rabbit antibody was made using a variable domain sequence of the original VHH format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | KF7 is highly specific for guinea pig C3, with no binding observed between KF7 and guinea pig C3a, rat C3 and human C3. |
| Application Notes: | ELISA was used to confirm the specificity of KF7 for guinea pig C3, with KF7 displaying high reactivity for gp C3. Reactivity with guinea pig C3a, rat C3, and human C3 was not observed. KF7 has a higher signal intensity than MF9 and MA11 (Hawlisch et al, 2000). SPR was used to quantify the dissociation rate constant of KF7; KF7 has a dissociation rate constant of 1.6x10^-4 s^-1 (Hawlisch et al, 2000). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |