Mfg Part Number
Ab01235-1.9
Vector Labs Ab01235-1.9 Anti-Complement C3 [MF9], Mouse IgG1-Fc fusion, His-tagged, (200 μg)
Quick Overview
A female New Zealand White rabbit was immunised with guinea pig C3 by three subsequent subcutaneous injections. The RNA was isolated from the spleen, a phage display library constructed and then used to produce soluble scFv fragments.
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| Product Class: | Purified |
|---|---|
| Clone ID: | MF9 |
| Heavy Chain Modification: | His-tagged |
| Synonyms: | C3 |
| Amount: | 200 μg |
| Application Codes Clone: | ELISA; IHC |
| Shipping Temperature: | Wet Ice |
| Origin Pub PMID: | 10699585 |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | scFv |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This chimeric mouse antibody was made using a variable domain sequence of the original VHH format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | MF9 is highly specific for guinea pig C3, with no binding observed between MF9 and guinea pig C3a, rat C3 and human C3. |
| Application Notes: | ELISA was used to confirm the specificity of MF9 for guinea pig C3, with MF9 displaying high reactivity for gp C3. Reactivity with guinea pig C3a, rat C3, and human C3 was not observed (Hawlisch et al, 2000). MF9 was used to stain a rejected transplanted liver in guinea pigs (Hawlisch et al, 2000). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |