Mfg Part Number
Ab01239-1.9
Vector Labs Ab01239-1.9 Anti-Calmodulin-domain protein kinase 1 [VHH-1B7], Mouse IgG1-Fc fusion, His-tagged, (200 μg)
Quick Overview
VHH phage display libraries from alpacas were pooled and panned against recombinant TgCDPK1, and from this clone 1B7 was selected.
$517.40
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| Product Class: | Purified |
|---|---|
| Clone ID: | VHH-1B7 |
| Heavy Chain Modification: | His-tagged |
| Synonyms: | CDPK1 |
| Amount: | 200 μg |
| Application Codes Clone: | ELISA; immunoprecipitation; crystallography |
| Shipping Temperature: | Wet Ice |
| Origin Pub PMID: | 26305940 |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | VHH |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This chimeric mouse antibody was made using a variable domain sequence of the original VHH format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | CDPKs regulate essential processes in parasites.VHH-IB7 recognises CDPK1 in a conformation-dependent manner and selectively inhibits the Ca^2+ bound form. It also recognises CDPK3 in a Ca2+-bound form. |
| Application Notes: | ELISA showed VHH-1B7 has reactivity against CDPK1. By immunoblot, 1B7 recognized both endogenous and recombinant versions of CDPK1 and its closest paralog CDPK3 (Ingram et al, 2015). Immunoprecipitation showed VHH-1B7 specifically recognises the Ca2+-bound form of CDPK1 and CDPK3. VHH-1B7 inhibits CDPK1 and CDPK3 with an IC50 of approximately 40 nm. The crystal structure of CDPK1 with VHH-1B7 showed VHH-1B7 interacts directly with the regulatory domain of CDPK1. |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |