Mfg Part Number
Ab01242-1.9
Vector Labs Ab01242-1.9 Anti-Capsid protein VP3 [VHH-12B], Mouse IgG1-Fc fusion, His-tagged, (200 μg)
Quick Overview
The VHH-12B was generated by immunizing a dromedary with poliovirus type I Sabin strain, preparing cDNA libraries from lymphocytes, and then selecting for tight poliovirus binders by phage display.
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| Product Class: | Purified |
|---|---|
| Clone ID: | VHH-12B |
| Heavy Chain Modification: | His-tagged |
| Amount: | 200 μg |
| Application Codes Clone: | cryo EM; ELISA |
| Shipping Temperature: | Wet Ice |
| Origin Pub PMID: | 27852863 |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | VHH |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This chimeric mouse antibody was made using a variable domain sequence of the original VHH format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | VHH-12B binds to a site on the top surface of the expanded poliovirion capsid protein VP3. VHH-12B does not bind to the native, unexpanded virus. |
| Application Notes: | ELISA was used to show the reactivity of VHH-12B for poliovirus (Thys et al, 2010). The interaction of VHH-12B with capsid protein VP3 has been captured using cryo electron microscopy (Strauss et al, 2017). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |