Mfg Part Number
Ab01244-10.9
Vector Labs Ab01244-10.9 Anti-Vesicular stomatitis virus nucleoprotein N [VHH-1004], Human IgG1-Fc fusion, (200 μg)
Quick Overview
The VHH was generated by immunising alpacas with inactivated vesicular stomatitis virus and extracting RNA from peripheral blood lymphocytes. From the extracted RNA a lentiviral plasmid VHH library was established and A549 cells transduced with lentiviral particles. The transduced A549 cells were then subject to lethal doses of VSV virus, and VHH-1004 was identified from this virus challenge.
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| Product Class: | Purified |
|---|---|
| Clone ID: | VHH-1004 |
| Synonyms: | VSV NP N |
| Amount: | 200 μg |
| Application Codes Clone: | FC; LUMIER assays; transcription assays |
| Shipping Temperature: | Wet Ice |
| Origin Pub PMID: | 28396572 |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | VHH |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This chimeric human antibody was made using a variable domain sequence of the original VHH format, for improved compatibility with existing reagents, assays and techniques. |
| Application Notes: | FC was used to confirm VHH-1004 prevents VSV infection of transformed A459 cells (Schmidt et al, 2016). LUMIER assays identified the antigenic target of VHH-1004 as VSV nucleoprotein N. Transcription assays showed that VHH-1004 prevents viral gene expression by blocking mRNA transcription (Schmidt et al, 2016). X-ray crystallography has captured the VHH-1004:Nucleoprotein N complex (Hanke et al, 2017). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |