Mfg Part Number
Ab01247-23.9
Vector Labs Ab01247-23.9 Anti-Norovirus P domain [Nano-4], Rabbit IgG-Fc fusion, His-Tagged, (200 μg)
Quick Overview
An alpaca was injected with norovirus GII.10 virus-like particles (VLPs), and a phage display library constructed. Nano-4 was selected from this phage display library.
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| Product Class: | Purified |
|---|---|
| Clone ID: | Nano-4 |
| Heavy Chain Modification: | His-Tagged |
| Amount: | 200 μg |
| Application Codes Clone: | ELISA; ITC; crystallography; neutralisation assay |
| Shipping Temperature: | Wet Ice |
| Origin Pub PMID: | 29095961 |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | VHH |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This chimeric rabbit antibody was made using a variable domain sequence of the original VHH format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | Nano-32 is specific for GII.10 strain of Norovirus, but shows limited cross-reactivity with other GI and GII norovirus strains, binding GII.10, GII.17, GII.12, and GII.1 P domains. |
| Application Notes: | The binding specificity of Nano-4 for the P domain of Norovirus strain GII.10 was confirmed using ELISA (Koromyslova et al, 2017). Nano-4 has stronger binding capabilities than Nano-27 or Nano-32; however, it also has limited cross-reactivity for other GI and GII norovirus strains. Nano-4 does not inhibit the binding of GII.10 to A-type saliva, B-type saliva, or pig gastric mucin as shown through neutralisation assays (Koromyslova et al, 2017). The crystal structure of Nano-4 in complex with the GII.17 P domain has been obtained (Koromyslova et al, 2017). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |