Mfg Part Number
Ab01253-10.3
Vector Labs Ab01253-10.3 Anti-ZIKV E domain III [Z004], Human IgG1, Fc Silent™, kappa (200 μg)
Quick Overview
Human serum was obtained from community participants in Mexico and Brazil and screened for ZIKV neutralisation activity, from which Z004 was identified.
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| Product Class: | Purified |
|---|---|
| Clone ID: | Z004 |
| Heavy Chain Modification: | Fc Silent™ |
| Synonyms: | ZEDIII |
| Amount: | 200 μg |
| Application Codes Clone: | ELISA; neutralisation assays; crystallography |
| Shipping Temperature: | Wet Ice |
| Origin Pub PMID: | 28475892 |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This reformatted human antibody was made using the variable domain sequences of the original Human IgG format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | Z004 is reactive towards ZIKV virus and shows cross-reactivity to DENV1. The epitope of Z004 is the lateral ridge of EDIII. |
| Application Notes: | Antibody binding activity to ZEDIII was measured by ELISA, and Z004 was found to have an EC50 of 23.7 ng/ml (Robbiani et al, 2017). ELISA also showed Z004 has cross-reactivity with the DENV1 virus (Robbiani et al, 2017). Neutralising assays established ZOO4 has an IC50 of 0.7 ng/ml for the ZIKV virus, the most potent antibody of those investigated (Robbiani et al, 2017). It also neutralises DENV1 . The structure of Z004 in complex with EDIII has been obtained, identifying the Z004 epitope as the EDIII lateral ridge. (Robbiani et al, 2017). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |