Mfg Part Number
Ab01255-10.0
Vector Labs Ab01255-10.0 Anti-ZIKV E domain II [SMZAb2], Human IgG1, lambda (200 μg)
Quick Overview
Antibody genes were cloned from blood-derived plasmablasts of a ZIKV-infected human subject from Colombia in the acute phase. Amplified heavy and light chains were tested for neutralisation, and SMZAb2 was selected from this.
$517.40
Order will ship within 1-3 business days
| Product Class: | Purified |
|---|---|
| Clone ID: | SMZAb2 |
| Synonyms: | ZEDII |
| Amount: | 200 μg |
| Application Codes Clone: | ELISA; therapeutic; neutralisation assays |
| Shipping Temperature: | Wet Ice |
| Origin Pub PMID: | 28978754 |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This reformatted human antibody was made using the variable domain sequences of the original Human IgG format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | SMZAb2 binds to domain II of the ZIKV E protein. This is distinct to the binding site of SMZAb5 and SMZAb1. |
| Application Notes: | ELISA was used to confirm SMZAb2 binds to the ZIKV E domain, and neutralisation assays identified the IC50 of SMZAb2 as 189 ng/ml. Four macaques were injected with a cocktail of anti-Zika antibodies (SMZAb1, SMZAb2, SMZAb5) and then infected with ZIKV virus. The antibody cocktail appeared to confer sterilising immunity on the treated macaques and completely prevented viral replication, which was not seen with the control group (Magnani et al, 2017). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |