Mfg Part Number
Ab01258-1.1
Vector Labs Ab01258-1.1 Anti-TIGIT [1B4], Mouse IgG1, lambda (200 μg)
Quick Overview
This antibody was raised by immunising TIGIT-/- mice with recombinant murine TIGIT tetramers.
$517.40
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| Product Class: | Purified |
|---|---|
| Clone ID: | 1B4 |
| Synonyms: | VSTM3; T cell Ig and ITIM domain; T cell immunoreceptor with Ig and ITIM domains; V-set and transmembrane domain-containing protein 3; WUCAM |
| Amount: | 200 μg |
| Application Codes Clone: | ELISA; FC; blocking |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG1 |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Specificity Statement: | This antibody is specific for murine TIGIT. |
| Application Notes: | The binding specificity of this antibody for TIGIT has been confirmed in ELISA analysis and by flow cytometry using P815 cells transfected with mouse TIGIT and activated mouse splenocytes (Dixon et al, 2018). This antibody also specifically stains activated primary wild-type T cells (Dixon et al, 2018). This antibody fully blocks CD155 binding to TIGIT, and acts as a TIGIT blocking antibody in vivo. Administration of this antibody increases experimental autoimmune encephalomyelitis (EAE) severity, and retards the growth of established MC38 colon carcinoma and GL261 glioblastoma (Dixon et al, 2018). This antibody synergises with anti-PD-1 blocking antibodies to achieve complete tumour regression in colon carcinoma models (Dixon et al, 2018). The original mouse IgG1 antibody does not deplete TIGIT-expressing CD4+ or CD8+ T cells in vivo, nor affect T cell proliferation in vitro (Dixon et al, 2018). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |