Vector Labs Ab01278-23.0 Anti-hINSR [IR 25-49], Rabbit IgG, kappa (200 μg) Centrifuge Bundle Savings at Sycamore Life Sciences

Description

Product Class: Purified
Clone ID: IR 25-49
Synonyms: CD220; human insulin receptor; insulin receptor; HHF5; IR; 25 49
Amount: 200 μg
Application Codes Clone: IP; blocking; stimulatory
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG1
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement: This confirmation-dependent antibody is specific for human INSR, reacting with an epitope within the Fn0 domain of the alpha subunit (amino acids 485-592). It cross reacts weakly with rabbit INSR, but not bovine, porcine, ovine, rat or mouse INSR.
Application Notes: This antibody has been used in immunoprecipitation assays, with radiolabelled human insulin receptor (hINSR) and hINSR-radiolabelled insulin complexes (Soos et al, 1986), and a dimeric soluble hINSR fragment containing the first four domains of the receptor (amino acids 1–593) (Surinya et al, 2002). It has also been used in immunoblot analysis with purified hINSR (Soos et al, 1986), revealing that it binds to the receptor’s alpha subunit. This antibody has been shown to cause >80% inhibition of insulin binding to its receptor, in assays with IM-9 cells (Soos et al, 1986), human adipocytes (Taylor et al, 1987) and NIH 3T3 fibroblasts transfected with hINSR cDNA (Soos et al, 1989). Additionally, this antibody has been shown to mimic the metabolic effects of insulin in serum-starved cells, stimulating 2-deoxyglucose uptake to 90% of the maximal insulin effect at 100nM, and thymidine incorporation to 30% of the maximal insulin effect (Soos et al, 1989). This insulin-mimetic antibody has also been shown to stimulate receptor kinase activity and receptor tyrosine phosphorylation in intact cells (Steele-Perkins et al, 1990; Brindle et al, 1990).

Product Class:	Purified
Clone ID:	IR 25-49
Synonyms:	CD220; human insulin receptor; insulin receptor; HHF5; IR; 25 49
Amount:	200 μg
Application Codes Clone:	IP; blocking; stimulatory
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG1
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:	This confirmation-dependent antibody is specific for human INSR, reacting with an epitope within the Fn0 domain of the alpha subunit (amino acids 485-592). It cross reacts weakly with rabbit INSR, but not bovine, porcine, ovine, rat or mouse INSR.
Application Notes:	This antibody has been used in immunoprecipitation assays, with radiolabelled human insulin receptor (hINSR) and hINSR-radiolabelled insulin complexes (Soos et al, 1986), and a dimeric soluble hINSR fragment containing the first four domains of the receptor (amino acids 1–593) (Surinya et al, 2002). It has also been used in immunoblot analysis with purified hINSR (Soos et al, 1986), revealing that it binds to the receptor’s alpha subunit. This antibody has been shown to cause >80% inhibition of insulin binding to its receptor, in assays with IM-9 cells (Soos et al, 1986), human adipocytes (Taylor et al, 1987) and NIH 3T3 fibroblasts transfected with hINSR cDNA (Soos et al, 1989). Additionally, this antibody has been shown to mimic the metabolic effects of insulin in serum-starved cells, stimulating 2-deoxyglucose uptake to 90% of the maximal insulin effect at 100nM, and thymidine incorporation to 30% of the maximal insulin effect (Soos et al, 1989). This insulin-mimetic antibody has also been shown to stimulate receptor kinase activity and receptor tyrosine phosphorylation in intact cells (Steele-Perkins et al, 1990; Brindle et al, 1990).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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