Mfg Part Number
Ab01281-10.3
Vector Labs Ab01281-10.3 Anti-hINSR [IR 83-22], Human IgG1, Fc Silent™, kappa (200 μg)
Quick Overview
This antibody was raised by immunising BALB/c mice with IM-9 lymphocytes, and boosting with purified human placental insulin receptor prior to fusion.
$517.40
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| Product Class: | Purified |
|---|---|
| Clone ID: | IR 83-22 |
| Heavy Chain Modification: | Fc Silent™ |
| Synonyms: | CD220; human insulin receptor; insulin receptor; HHF5; IR; 83 22 |
| Amount: | 200 μg |
| Application Codes Clone: | IP; IB; inhibitory |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG1 |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | This antibody is specific for human INSR, reacting with an epitope in the alpha subunit (amino acids 485-592). It does not cross-react with rabbit, rat or mouse insulin receptors, and displays very weak cross-reactivity with bovine, porcine and ovine receptors. |
| Application Notes: | This antibody has been used in immunoprecipitation assays, with both radiolabelled hINSR and hINSR-radiolabelled insulin complexes (Soos et al, 1986). It has also been used in immunoblot analysis with purified hINSR (Soos et al, 1986), revealing that it binds to the receptor’s alpha subunit. This antibody has been shown to cause >80% inhibition of insulin binding to IM-9 cells (Soos et al, 1986), and behaves as a surrogate agonist. |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |