Mfg Part Number
Ab01284-3.0
Vector Labs Ab01284-3.0 Anti-IGF1R [IGFR 16-13], Mouse IgG2b, lambda (200 μg)
Quick Overview
This antibody was raised by immunising BALB/c mice with transfected mouse fibroblasts expressing high levels of human IGF1R (IGF1R/3T3).
$517.40
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| Product Class: | Purified |
|---|---|
| Clone ID: | IGFR 16-13 |
| Synonyms: | CD221; IGF1 receptor; Insulin-like growth factor 1 receptor; type I IGF receptor; Insulin-like growth factor I receptor; IGF-I receptor; 6 13 |
| Amount: | 200 μg |
| Application Codes Clone: | IP; stimulatory |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG2b |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Specificity Statement: | This antibody is specific for the human insulin-like growth factor 1 receptor (IGF1R). It recognises the extracellular portion of the receptor, specifically a conformation-dependent epitope in the alpha subunit (amino acids 62-184). It does not cross-react with rat or mouse IGF1R or human insulin receptors in co-precipitation or immunodepletion assays. |
| Application Notes: | The ability of this antibody to bind human IGF1R has been confirmed in IGF1 binding assays using both IG1FR-overexpressing IGF1R/3T3 cells and soluble receptors (Soos et al, 1992). This antibody efficiently precipitates IGF1R solubilized from IGF1R/3T3 cells and receptor-IGF1 complexes (Soos et al, 1992). When immobilised, this antibody has been shown to remove 90% of all soluble IGF1R in immunodepletion experiments (Soos et al, 1992). It has also been used to capture IGF1R N-terminal fragments as part of a ligand plate binding assay (Molina et al, 2000). This antibody strongly stimulates both IGF1 and insulin binding to IGF1R/3T3 cells, and potentiates insulin stimulation of DNA synthesis (Soos et al, 1992). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |