Vector Labs Ab01290-13.12 Anti-IGF1R [IGFR 24-60], Human IgG4-S228P, kappa (200 μg) Centrifuge Bundle Savings at Sycamore Life Sciences

Description

Product Class: Purified
Clone ID: IGFR 24-60
Synonyms: IGF1 receptor; Insulin-like growth factor 1 receptor; type I IGF receptor; Insulin-like growth factor I receptor; IGF-I receptor; CD221; 24 60
Amount: 200 μg
Application Codes Clone: IP; inhibitory
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG2a
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG2a format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement: This antibody is specific for the human insulin-like growth factor 1 receptor (IGF1R). It recognises the extracellular portion of the receptor, specifically an epitope in the cysteine rich domain of the alpha subunit, between amino acids 184 and 283. It does not cross-react with rat or mouse IGF1R or human insulin receptors in co-precipitation or immunodepletion assays.
Application Notes: The ability of this antibody to bind human IGF1R has been confirmed in IGF1 binding assays using both IG1FR-overexpressing IGF1R/3T3 cells and soluble receptors (Soos et al, 1992). This antibody efficiently precipitates human IGF1R solubilised from IGF1R/3T3 cells (Soos et al, 1992) and human placental membranes (Bailyes et al, 1997), and has been used in immunoprecipitation analysis of 293 cells transfected with IGF1R or chimeric IGF1R-insulin receptor (IR) constructs (Schumacher et al, 1993). When immobilised, this antibody has been shown to remove essentially all soluble, IGF1R/3T3-derived IGF1R (Soos et al, 1992) and human placental membrane-derived IGF1R (Soos et al, 1993) in immunodepletion experiments. This antibody strongly inhibits IGF1 binding to its receptor (Soos et al, 1992), and has been shown to suppress IGF1-mediated stimulation of skin fibroblast proliferation (Okubo et al 2003).

Product Class:	Purified
Clone ID:	IGFR 24-60
Synonyms:	IGF1 receptor; Insulin-like growth factor 1 receptor; type I IGF receptor; Insulin-like growth factor I receptor; IGF-I receptor; CD221; 24 60
Amount:	200 μg
Application Codes Clone:	IP; inhibitory
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG2a
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG2a format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:	This antibody is specific for the human insulin-like growth factor 1 receptor (IGF1R). It recognises the extracellular portion of the receptor, specifically an epitope in the cysteine rich domain of the alpha subunit, between amino acids 184 and 283. It does not cross-react with rat or mouse IGF1R or human insulin receptors in co-precipitation or immunodepletion assays.
Application Notes:	The ability of this antibody to bind human IGF1R has been confirmed in IGF1 binding assays using both IG1FR-overexpressing IGF1R/3T3 cells and soluble receptors (Soos et al, 1992). This antibody efficiently precipitates human IGF1R solubilised from IGF1R/3T3 cells (Soos et al, 1992) and human placental membranes (Bailyes et al, 1997), and has been used in immunoprecipitation analysis of 293 cells transfected with IGF1R or chimeric IGF1R-insulin receptor (IR) constructs (Schumacher et al, 1993). When immobilised, this antibody has been shown to remove essentially all soluble, IGF1R/3T3-derived IGF1R (Soos et al, 1992) and human placental membrane-derived IGF1R (Soos et al, 1993) in immunodepletion experiments. This antibody strongly inhibits IGF1 binding to its receptor (Soos et al, 1992), and has been shown to suppress IGF1-mediated stimulation of skin fibroblast proliferation (Okubo et al 2003).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

Reviews