Mfg Part Number
Ab01293-2.0
Vector Labs Ab01293-2.0 Anti-IL-21R [ATR-107], Mouse IgG2a, lambda (200 μg)
Quick Overview
This antibody was isolated through phage display. The scFv parental clone (18A5), which binds human IL-21R, was isolated from the CS human scFv library, using BaF3 cells expressing human IL-21R and biotinylated IL-21R-Fc fusion protein. Four phage display libraries were constructed based on 18A5, and variants with increased affinity for both human and murine IL-21R were identified, including ATR-107.
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| Product Class: | Purified |
|---|---|
| Clone ID: | ATR-107 |
| Synonyms: | CD360; Ab01; Ab-01; ATR107; IL21R; Interleukin-21 receptor; IL-21 receptor; NILR; Novel interleukin receptor; Q9JHX3 |
| Amount: | 200 μg |
| Application Codes Clone: | neutralising; therapeutic |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | scFv |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This chimeric mouse antibody was made using the variable domain sequences of the original Human IgG1 format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | This antibody recognises both human and murine IL-21R extracellular domains, as both were used as targets during alternating rounds of phage display library panning. This antibody also binds to rat and cynomolgus monkey IL-21R, but does not cross-react with human IL-4Rα or IL-2Rβ. |
| Application Notes: | This antibody binds to both human and murine IL-21R; surface plasmon resonance analysis has revealed this antibody to have an affinity to human and cynomolgus monkey IL-21R of 2 nM, and to murine IL-21R of 16 nM (Vugmeyster et al, 2010). This antibody has been shown to neutralise the IL-21-dependent proliferation of both activated human primary B and CD4+ T cells and mouse CD8+ T cells (Vugmeyster et al, 2010). Additionally, this antibody inhibits IL-21-induced phosphorylation of STAT3 in CD4+ T cells in a concentration-dependent manner (Zhu et al, 2013). In the MRL-Faslpr mouse model of lupus, administration of this antibody has been shown to reduce anti-dsDNA antibodies and IgG deposits in the kidney (Vugmeyster et al, 2010). This antibody did not induce cytokine storm-associated gene activation in human PBMCs in vitro, nor when administered to cynomolgus monkeys (Guo et al, 2010). When administered to humans as part of a phase I trial, this antibody was highly immunogenic, with a prolonged pharmacodynamic effect, but was observed to lead to the development of anti-drug antibodies in 75% of subjects (Hua et al, 2013). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |