Mfg Part Number
Ab01295-23.0
Vector Labs Ab01295-23.0 Anti-LAG-3 [C9B7W], Rabbit IgG, kappa (200 μg)
Quick Overview
This antibody was raised by immunising Lewis rats with a murine LAG-3:Ig fusion protein.
$517.40
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| Product Class: | Purified |
|---|---|
| Clone ID: | C9B7W |
| Synonyms: | CD223; LAG3; Lymphocyte activation gene 3 protein |
| Amount: | 200 μg |
| Application Codes Clone: | FC; IP; blocking |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG1 |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This chimeric rabbit antibody was made using the variable domain sequences of the original Rat IgG1 format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | This antibody is specific for an epitope in the D2 domain of murine LAG-3. |
| Application Notes: | This antibody has been used in flow cytometric analysis of splenocytes (Workman et al, 2005), T cell hybridomas expressing wild‐type or chimeric CD4:murine LAG-3 (Workman et al, 2002) and CD4+ regulatory T cells (Chien et al, 2017). It has also been used in immunoprecipitation analysis of murine T hybridoma 3A9 cells transduced with murine LAG-3 (Li et al, 2004). This antibody blocks LAG-3 function in vitro, inhibiting antigen‐induced IL‐2 production in LAG-3-expressing T cell hybridomas, but does not block LAG-3:MHC class II interaction (Workman et al, 2002). Treatment with this antibody also blocks LAG-3 function in vivo, leading to accelerated homeostatic expansion of WT T cells (Workman et al, 2005). Administration of this LAG-3-blocking antibody, in combination with PD-L1 blockade, synergistically improves the survival of lymphodepleted myeloma-bearing mice (Jing et al, 2015). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |