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Mfg Part Number

Ab01310-1.1

Vector Labs Ab01310-1.1 Anti-XRCC3 [10F1/6], Mouse IgG1, kappa (200 μg)

VECTOR LABS

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Quick Overview

This antibody was raised by immunising mice with the denaturated recombinant human XRCC3-His6 protein which had been purified from 2.4 liters of E. coli FB810 recA− pLysS carrying pXRCC3-28c.

$517.40

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Description

Product Class: Purified
Clone ID: 10F1/6
Synonyms: X-Ray Repair Cross Complementing 3; X-ray repair cross-complementing protein 3; DNA repair protein XRCC3; X-ray repair complementing defective repair in Chinese hamster cells 3; CMM6; 10F1
Amount: 200 μg
Application Codes Clone: WB; ICC/IF
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG1
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Specificity Statement: This antibody is specific for XRCC3 and does not cross-react with Rad51B, Rad51C, Rad51D, Rad51, or XRCC2 in Western blot analysis. XRCC3 is a protein involved in the homologous recombination repair (HRR) pathway in chromosomal fragmentations and deletions.
Application Notes: WB: XRCC3 10F1/6 antibody was used to visualise XRCC3 on Western blots in multiple experiments (e.g. after previous gel filtration of infected pf9 cells; after immunoprecipitation with different antibodies and others) in a study on the RAD51 paralogs' complexes and their role in genetic recombination (Masson et al., 2001). WB: Sage et al. (2010) used this antibody to visualise XRCC3 protein on Western blots from lysates of HCT116 cells in the experiments demonstrating engagement of Rad51 proteins in mtDNA maintenance. ICC/IF: XRCC3 protein was immunostained with XRCC3 10F1/6 antibody in HEK293 cells (some of the cells had been subjected to the ionising radiation) in an experiment investigating the exact role of XRCC3 in homologous recombination (Forget et al., 2004).

Product Class:	Purified
Clone ID:	10F1/6
Synonyms:	X-Ray Repair Cross Complementing 3; X-ray repair cross-complementing protein 3; DNA repair protein XRCC3; X-ray repair complementing defective repair in Chinese hamster cells 3; CMM6; 10F1
Amount:	200 μg
Application Codes Clone:	WB; ICC/IF
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG1
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Specificity Statement:	This antibody is specific for XRCC3 and does not cross-react with Rad51B, Rad51C, Rad51D, Rad51, or XRCC2 in Western blot analysis. XRCC3 is a protein involved in the homologous recombination repair (HRR) pathway in chromosomal fragmentations and deletions.
Application Notes:	WB: XRCC3 10F1/6 antibody was used to visualise XRCC3 on Western blots in multiple experiments (e.g. after previous gel filtration of infected pf9 cells; after immunoprecipitation with different antibodies and others) in a study on the RAD51 paralogs' complexes and their role in genetic recombination (Masson et al., 2001). WB: Sage et al. (2010) used this antibody to visualise XRCC3 protein on Western blots from lysates of HCT116 cells in the experiments demonstrating engagement of Rad51 proteins in mtDNA maintenance. ICC/IF: XRCC3 protein was immunostained with XRCC3 10F1/6 antibody in HEK293 cells (some of the cells had been subjected to the ionising radiation) in an experiment investigating the exact role of XRCC3 in homologous recombination (Forget et al., 2004).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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